Producing single-cell protein (SCP) using an underutilized side-stream from green biorefineries, brown juice (BJ), addresses global food security and environmental challenges. In this work, BJ from a green biorefinery was investigated as a fermentation medium for cultivating the SCP yeast Cyberlindnera jadinii. Effects of C/N ratio, BJ concentration, and maceration methods on yeast growth and yields were analyzed. Results showed C. jadinii could not utilize pentoses within 24 h, reducing protein yield.
